Horseradish peroxidase (HRP) was biotinylated with biotinamidocaproate N-hydroxysuccinimide ester (BcapNHS) in a controlled manner to obtain biotinylated horseradish peroxidase (Bcap-HRP) with two biotin moieties per enzyme molecule. Avidin-mediated immobilization of HRP was achieved by first coupli
Immobilization of horseradish peroxidase by entrapment in natural polysaccharide
β Scribed by Sachin P. Shukla; Kalpana Modi; P. K. Ghosh; Surekha Devi
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 213 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0021-8995
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Immobilization of horseradish peroxidase on magnetite and removal of chlorophenols using immobilized enzyme were investigated. Immobilization by physical adsorption on magnetite was much more effective than that by the crosslinking method, and the enzyme was found to be immobilized at 100% of retain
Tissues from 25 female and 2 male patients of mammary carcinoma were examined by the horseradish--anti-horseradish peroxidase complex to localize the presence of estrogen. Carcinoma cells of 10 females and 2 male patients showed positive staining by this technique. The nucleus and cytoplasmic membra