H NMR (400 MHz, CDCl 3 ): d ยผ 9.53 (s, 3H, COOH), 7.17 (s, 1H, amide), 3.72-3.57 [m, 21H, SCH 2 CH 2 O, (CH 2 CH 2 O) 3 , OCH 2 CH 2 CONH, CH 2 CH(COOH), N(CH 2 COOH) 2 ], 3.21 (s, 2H, NHCH 2 ), 3.07 (t, 2H, SCH 2 CH 2 O), 2.49 (s, 2H, OCH 2 CH 2 CO), 2.30 (s, 3H, acetyl), 1.84 [s, 1H, CH 2 CH(COOH)
โฆ LIBER โฆ
Immobilization of His-Tagged Proteins on Various Solid Surfaces Using NTA-Modified Chitosan
โ Scribed by Oshige, Masahiko; Yumoto, Kazuhiro; Miyata, Hidefumi; Takahashi, Shunsuke; Nakada, Masaki; Ito, Kanako; Tamegai, Miwa; Kawaura, Hiroki; Katsura, Shinji
- Book ID
- 120279710
- Publisher
- Scientific Research Publishing
- Year
- 2013
- Weight
- 434 KB
- Volume
- 03
- Category
- Article
- ISSN
- 2165-6681
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Immobilization of His-Tagged Endoglucana
โ
Itsuko Nakamura; Akira Makino; Masashi Ohmae; Shunsaku Kimura
๐
Article
๐
2010
๐
John Wiley and Sons
๐
English
โ 406 KB
Reversible oriented immobilization of hi
โ
Thomas A. Keller; Claus Duschl; Dietmar Krรถger; Anne-Franรงoise Sรฉvin-Landais; Ho
๐
Article
๐
1995
๐
Elsevier Science
๐
English
โ 742 KB
Controlling the orientation of immobiliz
โ
Sufi R. Ahmed; Alexander B. Kelly; Timothy A. Barbari
๐
Article
๐
2006
๐
Elsevier Science
๐
English
โ 271 KB
One-step purification, covalent immobili
โ
Cesar Mateo; Gloria Fernรกndez-Lorente; Estrella Cortรฉs; Josรฉ L. Garcia; Roberto
๐
Article
๐
2001
๐
John Wiley and Sons
๐
English
โ 482 KB
## Abstract Epoxy supports covalently immobilize proteins following a twoโstep mechanism; that is, the protein is physically adsorbed and then the covalent reaction takes place. This mechanism has been exploited to combine the selectivity of metal chelate affinity chromatography with the covalent i
Quartz Crystal Microbalance (QCM): Usefu
โ
Sha, Xue; Sun, Chengjun; Xu, Xiaohe; Alexander, Laura; Loll, Patrick J.; Penn, L
๐
Article
๐
2012
๐
American Chemical Society
๐
English
โ 580 KB