Immobilization of His-Tagged Endoglucanase on Gold via Various Ni-NTA Self-Assembled Monolayers and Its Hydrolytic Activity
✍ Scribed by Itsuko Nakamura; Akira Makino; Masashi Ohmae; Shunsaku Kimura
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 406 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1616-5187
No coin nor oath required. For personal study only.
✦ Synopsis
H NMR (400 MHz, CDCl 3 ): d ¼ 9.53 (s, 3H, COOH), 7.17 (s, 1H, amide), 3.72-3.57 [m, 21H, SCH 2 CH 2 O, (CH 2 CH 2 O) 3 , OCH 2 CH 2 CONH, CH 2 CH(COOH), N(CH 2 COOH) 2 ], 3.21 (s, 2H, NHCH 2 ), 3.07 (t, 2H, SCH 2 CH 2 O), 2.49 (s, 2H, OCH 2 CH 2 CO), 2.30 (s, 3H, acetyl), 1.84 [s, 1H, CH 2 CH(COOH)), 1.68 (s, 1H, CH 2 CH(COOH)], 1.50 (s, 4H, CONHCH 2 CH 2 CH 2 CH 2 ). MS (FAB, matrix; NBA/DTT with oxalic acid): m/z ¼ 591.2 (calcd. for C 23 H 40 N 2 O 12 SNa [
P8-NTA(SAc)
A solution of S-acetyl dPEG TM 8 NHS ester (Quanta Biodesign, 100 mg, 0.17 mmol) in anhydrous DMSO (1 mL) was treated as described above to afford P8-NTA(SAc) (93.6 mg, 0.13 mmol). 1 H NMR (400 MHz, CDCl 3 ) d ¼ 9.49 (s, 3H, COOH), 7.15 (s, 1H, amide), 3.81-3.55 [m, 37H, SCH 2 CH 2 O, (CH 2 CH 2 O) 7 , OCH 2 CH 2 CONH, CH 2 CH(COOH), N(CH 2 COOH) 2 ], 3.19 (s, 2H, NHCH 2 ), 3.06 (t, 2H, SCH 2 CH 2 O), 2.47 (s, 2H, OCH 2 CH 2 CO), 2.31 (s, 3H, acetyl), 1.82 [s, 1H, CH 2 CH(COOH)], 1.64 [s, 1H, CH 2 CH(COOH)], 1.48 (s, 4H, CONHCH 2 CH 2 CH 2 CH 2 ). MS (FAB, matrix; NBA/DTT with oxalic acid): m/z ¼ 745.3 (calcd. for C 31 H 57 N 2 O 16 S [(M þ H) þ ] m/z ¼ 745.3).