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Immobilization of factor XIII on collagen membranes

✍ Scribed by Blanchy, B. G. ;Coulet, P. R. ;Gautheron, D. C.

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 642 KB
Volume: 20
Category: Article
ISSN: 0021-9304
DOI: 10.1002/jbm.820200404

No coin nor oath required. For personal study only.

✦ Synopsis

Factor XI11 from placenta was successfully grafted onto collagen membranes by the acyl-azide procedure. The transamidase activity retained on collagen membranes was determined by measuring the increase of fluorescence resulting from dansylcadaverine incorporation into casein. We studied the effect of different factors on the grafting: concentration and composition of the factor XI11 preparation in the coupling solution and influence of the preactivation of factor XI11 by thrombin. Stability studies have shown that the activity of factor XI11 grafted on collagen membranes was almost constant over a period of 8 months. Sterilization by y-irradiation of factor XI11 revealed a drastic loss of activity while the use of high-energized electron bombardment caused a reduced loss of activity. The potential of such a material for biomedical use is presently under investigation.

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