DNA immobilized onto an acyl-azide derivative of collagen membranes for use as immunoadsorbent
✍ Scribed by Raphaelle El Habib; Pierre R. Coulet; Kamel Sanhadji; Danielle C. Gautheron; Maurice Laville; J. Traeger
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 393 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
Single‐ and double‐stranded DNA were immobilized on films of highly polymerized collagen by a covalent process. The coupling was efficient at an acidic pH with an optimum at pH 5, while preventing the collagen film from any damage. In addition, no leakage occurred, so it was possible to use this DNA‐coated collagen film as an immunoadsorbent. Therefore the findings reported here suggest that the acyl‐azide procedure is also suitable for DNA binding on a proteinic support. Promising results in specific clearance of DNA antibodies were obtained in vitro.