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Immobilization of enzymes on heterofunctional epoxy supports

โœ Scribed by Mateo, Cesar; Grazu, Valeria; Palomo, Jose M; Lopez-Gallego, Fernando; Fernandez-Lafuente, Roberto; Guisan, Jose M

Book ID
109946140
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
718 KB
Volume
2
Category
Article
ISSN
1750-2799

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## Abstract Epoxy supports covalently immobilize proteins following a twoโ€step mechanism; that is, the protein is physically adsorbed and then the covalent reaction takes place. This mechanism has been exploited to combine the selectivity of metal chelate affinity chromatography with the covalent i