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Stabilization of enzymes by multipoint immobilization of thiolated proteins on new epoxy-thiol supports

✍ Scribed by Valeria Grazú; Olga Abian; Cesar Mateo; Franciso Batista-Viera; Roberto Fernández-Lafuente; José Manuel Guisán

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
721 KB
Volume
90
Category
Article
ISSN
0006-3592

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## Abstract Epoxy supports covalently immobilize proteins following a two‐step mechanism; that is, the protein is physically adsorbed and then the covalent reaction takes place. This mechanism has been exploited to combine the selectivity of metal chelate affinity chromatography with the covalent i