Immobilization of proteins on aldehyde-a
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Morris B. Fiddler; Gary R. Gray
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Article
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1978
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Elsevier Science
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English
β 560 KB
A method has been developed for the immobilization of proteins on derivatized polyacrylamide gels. Aminoethyl Bio-Gel P-I50 was converted to its stable N-2,3dihydroxypropyl derivative by borohydride reduction of the Schiff base formed with glyceraldehyde. Periodate oxidation of the modified gel prov