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Immobilization and Stabilization of Recombinant Multimeric Uridine and Purine Nucleoside Phosphorylases from Bacillus subtilis

✍ Scribed by Rocchietti, Silvia; Ubiali, Daniela; Terreni, Marco; Albertini, Alessandra M.; Fernández-Lafuente, Roberto; Guisán, José M.; Pregnolato, Massimo

Book ID
118255094
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
119 KB
Volume
5
Category
Article
ISSN
1525-7797

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✍ Daniela Ubiali; Silvia Rocchietti; Francesca Scaramozzino; Marco Terreni; Alessa 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 122 KB

## Abstract Multimeric uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP) of __Bacillus subtilis__ have been expressed from genes cloned in __Escherichia coli__, purified, characterized, immobilized and stabilized on solid support. A new immobilization strategy has been developed