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Immobilization and stabilization of horseradish peroxidase isoforms

โœ Scribed by Shahid Husain; Farahdiba Jafri; M. Saleemuddin


Book ID
111742098
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
464 KB
Volume
40
Category
Article
ISSN
1521-6543

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Horseradish peroxidase (HRP) was biotinylated with biotinamidocaproate N-hydroxysuccinimide ester (BcapNHS) in a controlled manner to obtain biotinylated horseradish peroxidase (Bcap-HRP) with two biotin moieties per enzyme molecule. Avidin-mediated immobilization of HRP was achieved by first coupli