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Immobilisierung von polymergebundenen oligodesoxyribocytidylsäuren an DEAE-cellulose

✍ Scribed by Schott, Herbert ;Watzlawick, Hildegard

Book ID
102938089
Publisher
Wiley (John Wiley & Sons)
Year
1981
Weight
670 KB
Volume
182
Category
Article
ISSN
0025-116X

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✦ Synopsis

Abstract

N‐Anisoyl‐deoxyribocytidine‐5′‐phosphate is chemically polycondensed. From the polycondensate, oligomers containing four or more monomeric units are isolated chromatographically and subsequently linked to a soluble polyvinylalcohol. After cleavage of the nucleobaseprotecting groups, the unreacted oligodesoxyribocytidylic acids are separated from polyvinylalcohol bound oligomers through ultrafiltration. DEAE‐Cellulose is subsequently coated with this polymer‐bound oligodeoxyribocytidylic acids. The now obtained PVAL‐p(dC)~n~‐DEAE‐Cellulose acts as a specific sorbent in template‐chromatography.

📜 SIMILAR VOLUMES

Immobilisierung von Oligodesoxyriboguany
Immobilisierung von Oligodesoxyriboguanylsäuren an vernetztem Polyvinylalkohol
✍ Schott, Herbert ;Watzlawick, Hildegard 📂 Article 📅 1981 🏛 Wiley (John Wiley & Sons) ⚖ 602 KB

## Abstract Protected homologs of deoxyriboguanylic acid are synthesized on a preparative scale by condensation of monomers according to the diester method. Oligomers with four and more monomeric units are isolated and bound to the free hydroxyl groups of an insoluble poly(vinylalcohol) gel via est