✦ LIBER ✦

Imaging rat esophagus using combination of reflectance confocal and multiphoton microscopy

✍ Scribed by S.M. Zhuo; J.X. Chen; X.S. Jiang; K.C. Lu; S.S. Xie

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 294 KB
Volume: 5
Category: Article
ISSN: 1612-2011
DOI: 10.1002/lapl.200810034

No coin nor oath required. For personal study only.

✦ Synopsis

We combine reflectance confocal microscopy (RCM) with multiphoton microscopy (MPM) to image rat esophagus. The two imaging modalities allow detection of layered-resolved complementary information from esophagus. In the keratinizing layer, the keratinocytes boundaries can be characterized by RCM, while the keratinocytes cytoplasm (keratin) can be further imaged by multiphoton autofluorescence signal. In the epithelium, the epithelial cellular boundaries and nucleus can be detected by RCM, and MPM can be used for imaging epithelial cell cytoplasm and monitoring metabolic state of epithelium. In the stroma, multiphoton autofluorescence signal is used to image elastin and second harmonic generation signal is utilized to detect collagen, while RCM is used to determine the optical property of stroma. Overall, these results suggest that the combination of RCM and MPM has potential to provide more important and comprehensive information for early diagnosis of esophageal cancer. 50 µm cell boundary cell cytoplasm Image of the keratinocytes. Combination of the reflectance confocal and multiphoton autofluorescence imaging modes

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