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Imaging Coronary Artery Microstructure Using Second-Harmonic and Two-Photon Fluorescence Microscopy

✍ Scribed by Zoumi, Aikaterini; Lu, Xiao; Kassab, Ghassan S.; Tromberg, Bruce J.


Book ID
119988972
Publisher
Biophysical Society
Year
2004
Tongue
English
Weight
595 KB
Volume
87
Category
Article
ISSN
0006-3495

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Simultaneous two-photon excited Β―uorescence (TPF) and second-harmonic generation (SHG) imaging is demonstrated using a single femtosecond laser and a scanning microscope. This composite nonlinear microscopic technique was applied to imaging DNA and chromosomes, and it was shown that the two differen