✦ LIBER ✦

Imaging calcium dynamics in living plant cells and tissues

✍ Scribed by N.D. Read; P.S. Shacklock; M.R. Knight; A.J. Trewavas

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 774 KB
Volume: 17
Category: Article
ISSN: 1065-6995
DOI: 10.1006/cbir.1993.1049

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The central role of Ca^2+^ signalling in plants is now well established. Much of our recent research has been based on the premise that the direct demonstration of signal‐response coupling via Ca^2+^ requires the imaging or measurement of cytosolic free Ca^2+^ in living cells. Methods (confocal microscopy, fluorescence ratio imaging and photon counting imaging) which we use for imaging Ca^2+^ with fluorescent dyes or recombinant aequorin, are described. Approaches for using dyes are now routine for many plant cells. However, the imaging Ca^2+^ in whole tissues of plants genetically transformed with the aequorin gene is a very new development. We predict that this method, first employed in our laboratory, will bring about a revolution in our understanding of Ca^2+^ signalling at the multicellular level.

📜 SIMILAR VOLUMES

Confocal laser scanning microscopy of ca

Confocal laser scanning microscopy of calcium dynamics in living cells

✍ Stricker, Stephen A.; Whitaker, Michael 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 614 KB

Confocal laser scanning microscopy (CLSM) is widely used to monitor intracellular calcium levels in living cells loaded with calcium-sensitive fluorophores. This review examines the basic advantages and limitations of CLSM in in vivo imaging analyses of calcium dynamics. The benefits of utilizing ra

Magnetic susceptibility shift selected i

Magnetic susceptibility shift selected imaging (MESSI) and localized 1H2O spectroscopy in living plant tissues

✍ Kai Zhong; Xin Li; Yair Shachar-Hill; Francis Picart; Arnold Wishnia; Charles S. 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 223 KB

Methods for imaging the structure and fu

Methods for imaging the structure and function of living tissues and cells: 2. Fluorescence lifetime imaging

✍ Paul J. Tadrous 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 126 KB 👁 2 views

This second article in the series shows how ¯uorescence lifetime imaging allows natural biochemical and physiological properties of tissues to act as contrast agents and so provide a basis for distinguishing normal and diseased tissue components. When combined with methods for imaging through non-tr

Calcium channels and signal transduction

Calcium channels and signal transduction in plant cells

✍ Eva Johannes; James M. Brosnan; Dale Sanders 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 719 KB

An increasing number of studies indicate that changes in cytosolic free Ca2+ ([Ca"],) mediate specific types of signal transduction in plant cells. Modulation of [Ca2+], is likely to be achieved through changes in the activity of Ca2+ channels, which catalyse passive influx of Ca2+ to the cytosol fr

Automated detection and recognition of l

Automated detection and recognition of live cells in tissue culture using image cytometry

✍ Ingrid Spadinger; Steven S. S. Poon; Branko Palcic 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 751 KB

Protein Dynamics in Living Cells : Multi

Protein Dynamics in Living Cells : Multidimensional Imaging, Photo Perturbation and Automated Analysis in Modern Life Microscopy

✍ Charles Gueudry; Jean Salamero; Vincent Fraisier; François Amblard; Victor Racin 📂 Article 📅 2006 🏛 Wiley (John Wiley & Sons) ⚖ 290 KB

To illustrate the performance of our system, we have chosen to present results obtained on complex molecular systems based on two distinct XFP-tagged proteins expressed in HeLa cells, both mainly associated to the Golgi organelle. Even though dymeclin (1) and Rab6A (2) are