✦ LIBER ✦

Methods for imaging the structure and function of living tissues and cells: 2. Fluorescence lifetime imaging

✍ Scribed by Paul J. Tadrous

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 126 KB
Volume: 191
Category: Article
ISSN: 0022-3417
DOI: 10.1002/1096-9896(200007)191:3<229::aid-path623>3.0.co;2-b

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✦ Synopsis

This second article in the series shows how ¯uorescence lifetime imaging allows natural biochemical and physiological properties of tissues to act as contrast agents and so provide a basis for distinguishing normal and diseased tissue components. When combined with methods for imaging through non-transparent tissues and tomographic reconstruction it shows promise as a new optical biopsy technique. In addition to this, specially designed vital ¯uorescent probes of speci®c biochemical, secondary messenger and receptor activity in living cells may be imaged using FLIM. This is the youngest of the techniques covered in these review articles on imaging, the ®rst FLIM images of cells having been produced in 1994.

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