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Image processing approaches to biological three-dimensional electron microscopy

✍ Scribed by Gabor T. Herman; Roberto Marabini; José-María Carazo; Edgar Garduño; Robert M. Lewitt; Samuel Matej

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
371 KB
Volume
11
Category
Article
ISSN
0899-9457

No coin nor oath required. For personal study only.

✦ Synopsis

Electron microscopy is a powerful technique for imaging complex biological macromolecules in order to further the understanding of their functions. When combined with sufficiently careful sample preparation procedures that preserve the native structure of the macromolecules and with sophisticated image processing procedures, electron microscopy can lead to very informative estimates of the three-dimensional (3D) structures of the specimens under study. 3D reconstruction from electron microscopic data is achieving high goals and exceeding expectations unthinkable only a few years ago. However, there are still some areas where either not enough work has been invested or the work has not as yet been fruitful. We describe image processing approaches that shed further light on some of these difficult areas.

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