Multiparameter laser scanning cytometry has been applied to the automatic counting of probe spots and the simultaneous measurement of cellular DNA for fluorescence in situ hybridization (FISH) prepared specimens counterstained with propidium iodide. Relatively low resolution imaging, highly variable
Image analysis enhancement of the laser scanning cytometer
β Scribed by Gerrit Woltmann; Andrew J. Wardlaw; David A. Rew
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 330 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
β¦ Synopsis
The laser scanning cytometer offers a range of novel applications and the capacity for direct visual validation of experiments through sample analysis on a microscope slide. Linkage of the instrument to an image analysis system through standard connections and software enhances the capabilities of the instrument in image capture and manipulation. In this technical note, we describe a simple linkage between the LSC and the Kontron KS100 Image Analysis System, an example of a standard commercial image processing instrument.
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