IL-10 produced by activated human B cells regulates CD4+ T-cell activation in vitro

✍ Scribed by Jean-David Bouaziz; Sebastien Calbo; Maud Maho-Vaillant; Anne Saussine; Martine Bagot; Armand Bensussan; Philippe Musette

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 328 KB
• Volume: 40
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.201040673

✦ Synopsis

Abstract

IL‐10‐producing regulatory B cells have been identified in mice and shown to downregulate inflammation, making them potentially important for maintenance of tolerance. In this study, we isolated B cells from human blood and spleen, and showed that after a short period of ex vivo stimulation a number of these cells produced IL‐10. The IL‐10‐producing B cells did not fall within a single clearly defined subpopulation, but were enriched in both the memory (CD27^+^) and the transitional (CD38^high^) B‐cell compartments. Combined CpG‐B+anti‐Ig stimulation was the most potent IL‐10 stimulus tested. B cells stimulated in this way inhibited CD4^+^CD25^−^ T‐cell proliferation in vitro by a partially IL‐10‐dependent mechanism. These findings imply that manipulating IL‐10 production by human B cells could be a useful therapeutic strategy for modulating immune responses in humans.