Identification of ϵ-N-monomethyllysine and ϵ-N-trimethyllysine in rabbit skeletal myosin
✍ Scribed by W. Michael Kuehl; Robert S. Adelstein
- Book ID
- 118851474
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 363 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0006-291X
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A method for measurement of epsilon-N-trimethyllysine in human blood plasma and urine is described. An internal standard, delta-N-trimethylornithine, was added to plasma and urine specimens and the mixtures were deproteinized and/or hydrolyzed. Preliminary purification of epsilon-N-trimethyllysine a
Poly(trimethy1-L-lysine), [Lys(Mq)],, is converted from random coil to a-helix at about 1/30 of the NaC10, concentration required by poly(L-lysine), (Lys),. NaClO, generates turbidity in [Lys(Me),], at concentrations above that required for helix formation, and decreases turbidity above 1M NaC10,. T