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Identification of γ-carboxyglutamic acid in ovocalcin

✍ Scribed by G. Krampitz; H. Meisel; W. Witt-Krause

Publisher
Springer
Year
1980
Tongue
English
Weight
225 KB
Volume
67
Category
Article
ISSN
0028-1042

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We report the first direct method for the identification of the vitamin K-dependent Ca2+ binding amino acid, gamma-carboxyglutamic acid (Gla), in the sequencing of proteins. The carboxyl groups on the protein are first converted to methyl esters with methanolic HCl, a procedure that reduces the pola