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Identification of two essential histidine residues of ribonuclease T2 from Aspergillus oryzae

โœ Scribed by Yasushi KAWATA; Fumio SAKIYAMA; Fumiaki HAYASHI; Yoshimasa KYOGOKU


Book ID
115127657
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
707 KB
Volume
187
Category
Article
ISSN
1432-1327

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Stability of ribonuclease T2 from Asperg
โœ Yasushi Kawata; Kozo Hamaguchi ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› Cold Spring Harbor Laboratory Press ๐ŸŒ English โš– 491 KB

## Abstract The stability of ribonuclease T2 (RNase T2) from __Aspergillus oryzae__ against guanidine hydrochloride and heat was studied by using CD and fluorescence. RNase T2 unfolded and refolded reversibly concomitant with activity, but the unfolding and refolding rates were very slow (order of