## Abstract The stability of ribonuclease T2 (RNase T2) from __Aspergillus oryzae__ against guanidine hydrochloride and heat was studied by using CD and fluorescence. RNase T2 unfolded and refolded reversibly concomitant with activity, but the unfolding and refolding rates were very slow (order of
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Amino-acid sequence of ribonuclease T2 from Aspergillus oryzae
โ Scribed by Yasushi KAWATA; Fumio SAKIYAMA; Hidetsune TAMAOKI
- Book ID
- 115125977
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 383 KB
- Volume
- 176
- Category
- Article
- ISSN
- 1432-1327
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Using synthetic oligonucleotide probes, we have cloned a genomic DNA sequence encoding a ribonuclease (RNase) T2 gene (rntB) from Aspergillus oryzae on a 4.8 kb HindIII fragment. DNA sequence analysis of the RNase T2 revealed the following: (1) The gene is arranged as five exons and four introns; (2