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Identification of trypsin-like activity in sturgeon spermatozoa

✍ Scribed by Ciereszko, Andrzej ;Dabrowski, Konrad ;Lin, Feng ;Doroshov, Serge I.

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
621 KB
Volume
268
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

We documented amidase activity in lake sturgeon (Acipenser fulvescens) and white sturgeon (Acipenser transmontanus) spermatozoa. This activity was inhibited by benzamidine and soybean trypsin inhibitor (SBTI), and N‐α‐p‐tosyl‐L‐lysine chloromethyl ketone‐HCl (TLCK), but not by N‐tosyl‐L‐phenylalanine chloromethyl keton (TPCK), indicating the presence of trypsin‐like activity in sturgeon spermatozoa. Higher activity occurred at pH 8.5 and 9.0 than at 8.0. No effect of 10 mM KCl and 100 mM NaCl on trypsin‐like activity was found. Calcium ions inhibited the activity at 10 and 50 mM. Similar activities were documented in neat semen and washed sperm suspension. Presence of trypsin‐like activity corresponds to the presence of acrosomes and acrosome reaction in spermatozoa of these fishes. © 1994 Wiley‐Liss, Inc.

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