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Identification of transforming activity of free fatty acid receptor 2 by retroviral expression screening

✍ Scribed by Hisashi Hatanaka; Mamiko Tsukui; Shuji Takada; Kentaro Kurashina; Young Lim Choi; Manabu Soda; Yoshihiro Yamashita; Hidenori Haruta; Toru Hamada; Toshihide Ueno; Kiichi Tamada; Yoshinori Hosoya; Naohiro Sata; Yoshikazu Yasuda; Hideo Nagai; Kentaro Sugano; Hiroyuki Mano


Book ID
108586530
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
687 KB
Volume
101
Category
Article
ISSN
1347-9032

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To identify transforming genes in acute myeloid leukemia (AML) we here constructed a retroviral cDNA expression library from an AML patient, and then used this library to infect a mouse cell line 32Dcl3-mCAT. cDNA inserts of the cell clones which proliferated in the presence of granulocyte colony-st