Identification of a constitutively active mutant of JAK3 by retroviral expression screening

✍ Scribed by Young Lim Choi; Ruri Kaneda; Tomoaki Wada; Shin-ichiro Fujiwara; Manabu Soda; Hideki Watanabe; Kentaro Kurashina; Hisashi Hatanaka; Munehiro Enomoto; Shuji Takada; Yoshihiro Yamashita; Hiroyuki Mano

• Book ID: 104040178
• Publisher: Elsevier Science
• Year: 2007
• Tongue: English
• Weight: 574 KB
• Volume: 31
• Category: Article
• ISSN: 0145-2126
• DOI: 10.1016/j.leukres.2006.05.006

✦ Synopsis

To identify transforming genes in acute myeloid leukemia (AML) we here constructed a retroviral cDNA expression library from an AML patient, and then used this library to infect a mouse cell line 32Dcl3-mCAT. cDNA inserts of the cell clones which proliferated in the presence of granulocyte colony-stimulating factor were derived from JAK3 encoding a JAK3 mutant with a valine-to-alanine substitution at codon 674 and two additional amino acid substitutions. The transforming activity of JAK3(V674A) was confirmed by its introduction into 32Dcl3-mCAT. Sequencing of the original JAK3 cDNA derived from the patient, however, failed to detect the V674A mutation.