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Identification of the tetrasaccharide repeating-unit of the Streptococcus pneumoniae type 23 polysaccharide by high-field proton n.m.r. spectroscopy

✍ Scribed by Christopher Jones


Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
560 KB
Volume
139
Category
Article
ISSN
0008-6215

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✦ Synopsis


One-and two-dimensional XIO-MHz 'H-n.m.r. studies of the capsular polysaccharide from Streptococcus pneumoniae type 23 have been used to determine its structure, which was confirmed by limited degradation. The proposed structure (1) differs from those suggested previously'J and the phosphate group has been located tentatively at position 3 of the glucosyl residue.


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The 1H-n.m.r. spectra of the Streptococcus pneumoniae type 9V (S68 in American nomenclature) capsular polysaccharide (PS) and its O-deacetylated derivative [which is structurally identical to the S. pneumoniae type 9A (S33) PS] were assigned using COSY, relayed-COSY, and 2D-NOESY experiments. The po