Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product
โ Scribed by Maria Cristina Thaller; Serena Schippa; Alessandra Bonci; Stefania Cresti; Gian Maria Rossolini
- Book ID
- 109321502
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 562 KB
- Volume
- 146
- Category
- Article
- ISSN
- 0378-1097
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The AphA enzyme of Escherichia coli, a molecular class B periplasmic phosphatase that belongs to the DDDD superfamily of phosphohydrolases, was purified and subjected to biochemical characterization. Kinetic analysis with several substrates revealed that the enzyme essentially behaves as a broad-spe
The lkyB gene of Escherichia coli K12 has been cloned from the Clarke and Carbon colony bank by selecting a ColEa plasmid conferring cholic acid resistance to lkyB mutants. The lkyB gene was localized on hybrid plasmid pJC778 by analysis of mutated plasmids generated by Tn5 insertions. Restriction a