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Cloning of the lkyB (tolB) gene of Escherichia coli K12 and characterization of its product

✍ Scribed by Lazzaroni, Jean Claude ;Fognini-Lefebvre, Nicole ;Portalier, Raymond C.

Publisher: Springer
Year: 1986
Tongue: English
Weight: 493 KB
Volume: 204
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425511

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✦ Synopsis

The lkyB gene of Escherichia coli K12 has been cloned from the Clarke and Carbon colony bank by selecting a ColEa plasmid conferring cholic acid resistance to lkyB mutants. The lkyB gene was localized on hybrid plasmid pJC778 by analysis of mutated plasmids generated by Tn5 insertions. Restriction analysis and complementation studies indicated that plasmid pJC778 carried genes nadA, lkyB and sucA which mapped at min 16.5; the lkyB + allele was dominant over the lkyB207 mutant allele. Analysis of cell envelope proteins from strains carrying plasmids pjC778 (lkyB+), pjC2578 or pJC2579 (lkyB: :Tn5), as well as plasmid-coded proteins in a maxicell system, made it likely that the lkyB gene product was a membrane protein of molecular weight 42,000.

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