A fragment of the E. coli chromosome including the recC gene has been cloned by in vitro recombinant DNA techniques into a phage lambda vector to give the recombinant phage lambda drecC. This was used to derive the phage lambda drecBC by in vivo recombination. On lysogenisation of recB and recC muta
Identification of the Escherichia coli recB and recC gene products
β Scribed by Hickson, Ian D.; Emmerson, Peter T.
- Book ID
- 109725007
- Publisher
- Nature Publishing Group
- Year
- 1981
- Tongue
- English
- Weight
- 463 KB
- Volume
- 294
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/294578a0
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A specialized transducing lambda phage carrying the dnaN and dnaA genes of Escherichia coli specifies two proteins of about 41 and 48 kilodaltons (kd). The temperature-sensitive mutations, dnaN59 and dnaA167, were found to result in altered isoelectric points of the 41 and 48 kd proteins, respective
Escherichia coli nusB mutants fail to support the activity of a phage lambda gene product, pN, which regulates phage gene expression by influencing transcription termination. We report the identification of the nusB protein on SDS-polyacrylamide gels as a 14,500 dalton protein.