✦ LIBER ✦

Identification of Sulfonylureas in Serum by Electrospray Mass Spectrometry

✍ Scribed by F. Magni; L. Marazzini; S. Pereira; L. Monti; M.Galli Kienle

Book ID: 102562952
Publisher: Elsevier Science
Year: 2000
Tongue: English
Weight: 90 KB
Volume: 282
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.2000.4597

No coin nor oath required. For personal study only.

✦ Synopsis

Identification of sulfonylureas in serum is important in the diagnosis of hypoglycemic crisis of unknown origin. Methods based on HPLC with UV or fluorescence detection may give false positive results. Mass spectrometry may successfully avoid this problem. The described method allows the simultaneous identification and quantification of tolbutamide, chlorpropamide, glibenclamide, and glipizide in human serum using one of the tested sulfonylureas as the internal standard. Serum purification is carried out by solidphase extraction with ENVI-C18 cartridges and samples are analyzed by liquid chromatography-electrospray mass spectrometry. For all drugs, the limit of detection and the limit of quantification are about 2 and 10 ng/ml, respectively.

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