A simple, rapid, and reliable technique for identifying small amounts of phenylthiohydantoin (PTH) derivatives has been developed for use in the analysis by Edman degradation of small amounts of peptides isolated from fingerprints (10). Solvent systems used in paper and column chromatography of PTH
Identification of subnanomole amounts of PTH-amino acids by high performance thin-layer chromatography
β Scribed by D. Bucher
- Book ID
- 112720779
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 173 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0009-5893
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π SIMILAR VOLUMES
Thin-layer chromatography of PTH amino acids on polyamide sheets with a fluorescent indicator gave a 5&lC%fold increase in sensitivity when compared to thin-layer polyamide plates developed by spray techniques. Resolution of the individual residues in a st.andard mixture remains comparable to that d
## Identification of phenylthiohydantoins of amino acids-by thin-layer chromatography The, identification of amino acids as their phenylthiohydantoins (PTHs) is of major importance in sequence determinations of peptides and proteins by the EDMAN degradaiion technique. The need for a rapid procedur
## Separation of amino acids as derivatives of phenylthiohydantoin (PTEkmino acids) has been performed by paper and thin-layer chromatography, gas chromatography and high-performance liquid chromatography (HPLC). EIPLC, being a rapid and highly efficient technique, is advantageous because all PTH-