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Identification of placental alkaline phosphatase in human sera using polyacrylamide gel electrophoresis

✍ Scribed by Francoise Guilleux; Monique Hayer; Nicole Thomas; Bernard Magnan De Bornier


Book ID
115822141
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
725 KB
Volume
87
Category
Article
ISSN
0009-8981

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Polyacrylamide gel electrophoresis in the presence of 7 M urea provides a simple, reproducible method for the identification of cathodic alcohol dehydrogenase (ADH) isozymes. Treatment of native ADH dimers with 7 M urea and 1 mM dithiothreitol results in a complete dissociation of the 40,000 Mr subu