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Identification of novel genes expressed during rhabdomyosarcoma differentiation using cDNA microarrays

โœ Scribed by Kate A. Carey; David Segal; Reuben Klein; Andrew Sanigorski; Ken Walder; Gregory R. Collier; David Cameron-Smith


Book ID
115175641
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
347 KB
Volume
56
Category
Article
ISSN
1320-5463

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Molecular indexing is a technique to select a subpopulation of cDNA by ligation of adapters to cDNA fragments digested by a class IIS restriction enzyme(s). By this technique, 3' end cDNA fragments are divided into 16 fractions by selective ligation of adapters and subsequent PCR amplification. Each