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Identification of differentially expressed genes in salivary gland tumors with cDNA microarray

โœ Scribed by Kazuyuki Kainuma; Satoshi Katsuno; Shigenari Hashimoto; Nobuyoshi Suzuki; Tomohiro Oguchi; Kenji Asamura; Kosei Nakajima; Shin-ichi Usami


Book ID
116223139
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
689 KB
Volume
31
Category
Article
ISSN
0385-8146

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Molecular indexing is a technique to select a subpopulation of cDNA by ligation of adapters to cDNA fragments digested by a class IIS restriction enzyme(s). By this technique, 3' end cDNA fragments are divided into 16 fractions by selective ligation of adapters and subsequent PCR amplification. Each