Microarray Hybridization with Fractionat
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Kohji Sakai; Hirofumi Higuchi; Kenichi Matsubara; Kikuya Kato
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Article
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2000
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Elsevier Science
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English
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Molecular indexing is a technique to select a subpopulation of cDNA by ligation of adapters to cDNA fragments digested by a class IIS restriction enzyme(s). By this technique, 3' end cDNA fragments are divided into 16 fractions by selective ligation of adapters and subsequent PCR amplification. Each