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Identification of Novel Centrosomal Proteins in Dictyostelium d iscoideum by Comparative Proteomic Approaches

✍ Scribed by Reinders, Yvonne; Schulz, Irene; Gräf, Ralph; Sickmann, Albert


Book ID
126829670
Publisher
American Chemical Society
Year
2006
Tongue
English
Weight
575 KB
Volume
5
Category
Article
ISSN
1535-3893

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We have set up a fast and easy methodology to identify cell-surface proteins in live yeasts. A non-gel proteomic approach was based on a short period of trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS. Candida albicans was used as a model