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Identification of messenger RNA substrates for mouse T-STAR

โœ Scribed by L. Y. Zhang; M. Zeng; P. Chen; H. Q. Sun; D. C. Tao; Y. Q. Liu; L. Lin; Y. Yang; S. Z. Zhang; Y. X. Ma


Book ID
110155267
Publisher
Springer
Year
2009
Tongue
English
Weight
241 KB
Volume
74
Category
Article
ISSN
0006-2979

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Specific messenger RNA for glutamate dehydrogenase was partially purified from a calf liver polysomal poly(A)-mRNA fraction by sucrose density gradient centrifugation. Enzyme activity in the translational incubation mixture was detected by measuring NADH oxidation in the presence of c~-ketoglutarate