A new mutant alpha-1-antichymotrypsin (variant ACT) was found by polymerase chain reaction single strand conformation polymorphism and direct sequencing. In this variant ACT, two bases (AA) were deleted from codon 391. This resulted in a different amino acid sequence downstream of the deletion point
Identification of HLA-C alleles using PCR—single-strand-conformation polymorphism and direct sequencing
✍ Scribed by H. Wang; K. Tokunaga; T. Akaza; K. Tadokoro; Y. Shibata; T. Juji
- Book ID
- 110890611
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 660 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0001-2815
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A total of 27 strains of Campylobacterjejuni (24 clinical strains and three laboratory strains) were examined for the presence of point mutations in the quinolone resistance determining region (QRDR) of gyrA gene by nonradioisotopic single-strand conformation polymorphism (non-RI SSCP) analysis with
Genetic diagnosis of an inherited disease or cancer often involves analysis for unknown point mutations in several genes; therefore, rapid and automated techniques that can process a large number of samples are needed. We describe a method for high-throughput single-strand conformation polymorphism