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Identification of Gram-negative bacteria by isoelectric focusing of soluble cellular proteins

✍ Scribed by Andreas Margull; Dr. Roland Süssmuth; Peter Schulz

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
348 KB
Volume
13
Category
Article
ISSN
0173-0835

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✦ Synopsis

Abstract

Strains of Escherichia coli. Pseudomonas aeruginosa and Proteus mirabilis, isolated from clinical specimens were disrupted by repeated freezing in liquid nitrogen and thawing at room temperature. The samples were separated by isoelectric focusing using polyacrylamide gels. The resulting protein patterns showed clear differences between the three species and made identification possible.

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