Identification of group specific component/vitamin D-binding protein (GC/DBP) mutants by isoelectric focusing in immobilized pH gradients
β Scribed by Jacques Constans; Prof. Dr. Hartwig Cleve
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 525 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0173-0835
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π SIMILAR VOLUMES
The group-specific component (GC) was discovered in 1959, and in the same year a vitamin D binding protein (DBP) in human plasma was found; however, their identity was established as late as 1975. In the GC/DBP system three common alleles, GC\*1F, GC\*1S, and GC\*2, determine six GC phenotypes: 1F,
A powerful method for group specific component (GC) subtyping with good resolution of GC bands by isoelectric focusing on ultrathin immobilized pH gradients is described. After separation, GC detection is achieved using a highly sensitive alkaline phosphatase conjugated enzyme-immuno system. The eff