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Identification of Fibrinogen-Binding Proteins ofAspergillus fumigatusUsing Proteomic Approach

✍ Scribed by Santosh Kumar Upadhyay; Poonam Gautam; Hrishikesh Pandit; Yogendra Singh; Seemi Farhat Basir; Taruna Madan

Publisher
Springer Netherlands
Year
2011
Tongue
English
Weight
362 KB
Volume
173
Category
Article
ISSN
0301-486X

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We have set up a fast and easy methodology to identify cell-surface proteins in live yeasts. A non-gel proteomic approach was based on a short period of trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS. Candida albicans was used as a model