Identification of disulfide bridges in a cardiotoxic peptide by electrospray ionization
β Scribed by Thaiya Krishnamurthy; Charles R. Hauer; M. Prabhakaran; James G. Freedy; Kyozo Hayashi
- Book ID
- 102773774
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 609 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1076-5174
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
In order to separate and characterize the target peptide and the side-product peptide compounds of a synthesis crude of the peptide hormone carbetocin, liquid chromatography coupled to high-flow electrospray ionization mass spectrometry (LC/ES-MS) has been used. Carbetocin is an important drug with
## Abstract Identifying the Cys residues involved in disulfide linkages of peptides and proteins that contain complex disulfide bond patterns is a significant analytical challenge. This is especially true when the Cys residues involved in the disulfide bonds are closely spaced in the primary sequen
## Abstract A simple and rapid method for counting the number of internal disulfide bridges in a protein by incubation with 2βmercaptoethanol and electrospray mass spectrometry analysis of the products was developed. 2βMercaptoethanol yields intermediate mixed disulfides during reduction of a prote