✦ LIBER ✦

Identification of antigenic differences between the phosphorylated and nonphosphorylated forms of the E7 protein of human papillomavirus type 16

✍ Scribed by Kee, Sun-Ho; Choi, Yong-Ok; Song, Yong-Sang; Lee, Hyo-Pyo; Chang, Woo-Hyun

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 316 KB
Volume: 54
Category: Article
ISSN: 0146-6615
DOI: 10.1002/(sici)1096-9071(199802)54:2<129::aid-jmv10>3.0.co;2-e

No coin nor oath required. For personal study only.

✦ Synopsis

To analyze the antigenic properties of the human papillomavirus type 16 E7 oncoprotein, two monoclonal antibodies, VD6 and IB10, that have different reactivities to the E7 protein were generated. While the VD6 antibody reacted strongly with E7 protein in CaSki cell extracts, the other antibody, IB10, showed much weaker reactivity with E7. This reactivity increased in a dosedependent manner in the presence of the casein kinase II-specific inhibitor DRB (5,6-dichloro-1beta-D-ribofuranosylbenzimidazole). Antigenic site estimation and an in vitro phosphorylation assay, using bacterially expressed E7 protein, demonstrated that the weak reactivity of IB10 was related to the phosphorylation status of the E7 protein. Phosphorylation of E7 reduced considerably the reactivity of IB10 but did not affect the reactivity of VD6, which reacts with the N-terminal portion of E7. In immunoprecipitation (IP) assays, IB10 precipitated weakly the E7 protein from CaSki cell extracts. Together, these data suggest that unphosphorylated E7 protein shows distinct antigenic character compared to its phosphorylated form under denaturing conditions; however, under native conditions, the phosphorylated and nonphosphorylated E7 proteins have some antigenic crossreactivity.

📜 SIMILAR VOLUMES

Human papillomavirus-16 E7 protein inhib

Human papillomavirus-16 E7 protein inhibits the DNA interaction of the TATA binding transcription factor

✍ Edio Maldonado; María Eugenia Cabrejos; Lawrence Banks; Jorge E. Allende 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 165 KB

## Abstract Previous studies have shown that the HPV‐16 E7 protein interacts with TBP. This interaction was found to take place through residues in the carboxy terminal half of E7, mutation of which resulted in weaker transforming activity. In addition, binding of E7 to TBP was found to be increase

Association of the antibodies against hu

Association of the antibodies against human papillomavirus 16 E4 and E7 proteins with cervical cancer positive for human papillomavirus DNA

✍ Takashi Onda; Tadahito Kanda; Soichi Zanma; Toshiharu Yasugi; Sumie Watanabe; Ta 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 French ⚖ 698 KB

Immortalization of normal human embryoni

Immortalization of normal human embryonic fibroblasts by introduction of either the human papillomavirus type 16 E6 or E7 gene alone

✍ Akito Yamamoto; Shin-ichi Kumakura; Minoru Uchida; J. Carl Barrett; Takeki Tsuts 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 French ⚖ 452 KB

## Abstract The ability of the human papillomavirus type 16 (HPV‐16) E6 or E7 gene to induce immortalization of normal human embryonic fibroblast WHE‐7 cells was examined. WHE‐7 cells at 9 population doublings (PD) were infected with retrovirus vectors encoding either HPV‐16 E6 or E7 alone or both

Mapping of linear epitopes of human papi

Mapping of linear epitopes of human papillomavirus type 16: The E1, E2, E4, E5, E6 and E7 open reading frames

✍ Joakim Dillner 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 French ⚖ 882 KB

Publication of the International Union Against Cancer Publication de I'Union lnternationale Contre 1e Cancer

Can a test for E6/E7 transcripts of huma

Can a test for E6/E7 transcripts of human papillomavirus type 16 serve as a diagnostic tool for the detection of micrometastasis in cervical cancer?

✍ Judit Czeglédy; Constantin Iosif; Bengt Göran Hansson; Magnus Evander; Lajos Ger 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 French ⚖ 767 KB

Tissue from I I cases of cervical cancer positive for human papillomavirus (HPV) type I 6 DNA and 69 pelvic lymph nodes from the same patients were examined for HPV 16 DNA and mRNA from the E6/E7 genes. Five of the tumors were squamom, 3 adeno-and 3 adenosquamous carcinoma. From the primary tumors a

Identification of an HLA-A24-restricted

Identification of an HLA-A24-restricted cytotoxic T lymphocyte epitope from human papillomavirus type-16 E6: The combined effects of bortezomib and interferon-γ on the presentation of a cryptic epitope

✍ Satoko Morishima; Yoshiki Akatsuka; Akihiro Nawa; Eisei Kondo; Tohru Kiyono; Hir 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 French ⚖ 579 KB

## Abstract About 50% of cervical cancers are associated with human papillomavirus type 16 (HPV‐16), and since the HPV‐16 E6 and E7 oncoproteins are constitutively expressed in the tumor cells, they are attractive targets for cytotoxic T lymphocyte (CTL)‐mediated immunotherapy. Nevertheless, only a