𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene

✍ Scribed by Rose, Mark ;Winston, Fred

Book ID
104747722
Publisher
Springer
Year
1984
Tongue
English
Weight
381 KB
Volume
193
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

Insertion mutations in yeast caused by the transposable element Ty have been identified at several genetic loci. In all cases so far, the site of Ty insertion has been in the 5' non-coding region of the affected gene. Experiments presented here demonstrate that the ura3-52 mutation, a non-reverting ura3 mutation, is caused by a Ty insertion mutation within the coding region of the URA3 gene. This Ty insertion within a gene has a similar structure to those in non-coding regions.

πŸ“œ SIMILAR VOLUMES

Inserted sequence in the mitochondrial 2
Inserted sequence in the mitochondrial 23S ribosomal RNA gene of the yeast Saccharomyces cerevisiae
✍ Faye, GοΏ½rard ;Dennebouy, Nicole ;Kujawa, Chantal ;Jacq, Claude πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 924 KB

The sequence organization of the yeast mit-DNA region carrying the large ribosomal RNA gene and the polar locus omega was examined. Hybridization studies using rho- deletion mutants and electron microscopy of the heteroduplexes formed between 23S rRNA and the appropriate restriction fragments, lead