𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Identification of a sperm protein 17 CTL epitope restricted by HLA-A1

✍ Scribed by Maurizio Chiriva-Internati; Zhiqing Wang; Stephanie Pochopien; Emanuela Salati; Seah H. Lim

Publisher
John Wiley and Sons
Year
2003
Tongue
French
Weight
70 KB
Volume
107
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Sperm protein 17 (Sp17) is a novel cancer‐testis antigen. We previously reported the successful generation of Sp17‐specific HLA‐A1‐restricted cytotoxic T‐lymphocytes (CTLs) from the peripheral blood of a healthy donor using a recombinant Sp17 protein. These CTLs were able to kill not only target cells pulsed with the recombinant protein but also fresh Sp17^+^ tumor cells. In the present study, we have identified a nonapeptide sequence within the Sp17 protein that is predicted to have a high binding affinity for the HLA‐A1 molecules. We generated the synthetic nonapeptide and successfully propagated a peptide‐specific CTL line. We confirmed that peptide Sp17~103–111~ (ILDSSEEDK) contains an Sp17 CTL epitope restricted by HLA‐A1 and identified amino acid residues 104, 107, 109 and 110 as crucial for the CTL lysis. Our findings therefore provide the tool for the characterization of CD8 T‐cell function in vivo and generation of epitope‐specific treatment strategies. © 2003 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Identification of HLA-A*0201-restricted
Identification of HLA-A*0201-restricted CTL epitopes encoded by the tumor-specific MAGE-2 gene product
✍ Marjan J.W. Visseren; Sjoerd H. van der Burg; Ellen I.H. van der Voort; Remco M. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 105 KB 👁 1 views

## MAGE -2 is expressed in many tumors, including melanoma, laryngeal tumors, lung tumors and sarcomas, but not in healthy tissue, with the exception of testis. Thus, MAGE-2derived peptides that bind to HLA class I molecules and elicit cytotoxic T lymphocyte (CTL) responses could be of significant

Identification of a new HLA-A*0201-restr
Identification of a new HLA-A*0201-restricted cryptic epitope from CYP1B1
✍ Britta Maecker; Michael S. von Bergwelt-Baildon; David H. Sherr; Lee M. Nadler; 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 100 KB

## Abstract Cytochrome P450 1B1 (CYP1B1) was recently shown to be a candidate tumor antigen broadly expressed in solid and hematologic malignancies. Nevertheless, use of such self‐antigens as targets for immune intervention can be limited because of loss of high‐avidity T cells during negative sele

HLA-A2-restricted CTL epitopes of a nove
HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL
✍ Michiko Harao; Shinya Hirata; Atsushi Irie; Satoru Senju; Tetsuya Nakatsura; Hir 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 French ⚖ 695 KB

## Abstract Toward the development of a novel cancer immunotherapy, we have previously identified several tumor‐associated antigens (TAAs) and the epitopes recognized by human histocompatibility leukocyte (HLA)‐A2/A24‐restricted cytotoxic T lymphocyte (CTL). In this study, we tried to identify a TA

Identification of a dengue virus-specifi
Identification of a dengue virus-specific HLA-A*0201-restricted CD8+ T cell epitope
✍ Jinsheng Wen; Zhiliang Duan; Lifang Jiang 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 229 KB

## Abstract In this study, a combination of epitope‐prediction programs and in vitro assays was used to identify dengue virus (DENV)‐specific CD8^+^ T cell epitopes. Peripheral blood mononuclear cells (PBMCs) isolated from patients who recovered from dengue fever were stimulated with candidate epit

Identification of a novel peptide derive
Identification of a novel peptide derived from the melanocyte-specific gp100 antigen as the dominant epitope recognized by an HLA-A2.1-restricted anti-melanoma CTL line
✍ Alexander B. H. Barker; Marco W. J. Schreurs; Gaalda Tafazzul; Annemiek J. De Bo 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 French ⚖ 633 KB

## Abstract Cytotoxic T lymphocytes (CTL) reactive with human melanoma tumor cells occasionally display cross‐reactivity with normal melanocytes. Previously, we identified the melanocyte lineage‐specific antigen gp100 that is expressed by both melanoma cells and normal melanocytes, as a target anti

Inducible Hsp70 as target of anticancer
Inducible Hsp70 as target of anticancer immunotherapy: Identification of HLA-A*0201-restricted epitopes
✍ Olivier Faure; Stéphanie Graff-Dubois; Laurent Bretaudeau; Laurent Derré; David- 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 French ⚖ 259 KB

## Abstract The design of a broad application tumor vaccine requires the identification of tumor antigens expressed in a majority of tumors of various origins. We questioned whether the major stress‐inducible heat shock protein Hsp70 (also known as Hsp72), a protein frequently overexpressed in huma