𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Identification of a dengue virus-specific HLA-A*0201-restricted CD8+ T cell epitope

✍ Scribed by Jinsheng Wen; Zhiliang Duan; Lifang Jiang

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
229 KB
Volume
82
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In this study, a combination of epitope‐prediction programs and in vitro assays was used to identify dengue virus (DENV)‐specific CD8^+^ T cell epitopes. Peripheral blood mononuclear cells (PBMCs) isolated from patients who recovered from dengue fever were stimulated with candidate epitope peptides derived from DENV, which were predicted by using SYFPEITHI and RANKpep epitope‐prediction programs. The IFN‐γ ELISpot results and the results of intracellular staining of IFN‐γ showed that peptides NS4b_40 (TLYAVATTI), E_256 (QEGAMHTAL), NS3_205 (LPAIVREAI), NS5_210 (SRNSTHEMY), and NS3_207 (AIVREAIKR) could induce the recall response of CD8^+^ T cells. Furthermore, the results of the MHC–peptide complex stabilization assay revealed that peptide NS4b_40 (TLYAVATTI) has a high affinity for HLA‐A*0201 molecules. The IFN‐γ ELISpot results and staining of intracellular IFN‐γ confirmed that this peptide could induce high‐level CD8^+^ T cell response in HLA‐A*0201 positive PBMCs. Peptide NS4b_40 (TLYAVATTI) was identified as a novel DENV‐specific HLA‐A*0201‐restricted CD8^+^ T cell epitope. J. Med. Virol. 82:642–648, 2010. © 2010 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Sequence flexibility of the immunodomina
Sequence flexibility of the immunodominant HLA A*0201 restricted ppUL83 CD8 T-cell epitope of human cytomegalovirus
✍ Jakub Kopycinski; Mohamed Osman; Paul D. Griffiths; Vincent C. Emery 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 194 KB

The cytomegalovirus ppUL83 protein contains an immunodominant A\*0201 restricted epitope between residues 495 and 503. We investigated the tolerance of this epitope to sequence variation in the context of peptide binding to HLA A\*0201 and the ability to induce an Interferon gamma (IFNgamma) respons

Identification of a new HLA-A*0201-restr
Identification of a new HLA-A*0201-restricted cryptic epitope from CYP1B1
✍ Britta Maecker; Michael S. von Bergwelt-Baildon; David H. Sherr; Lee M. Nadler; 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 100 KB

## Abstract Cytochrome P450 1B1 (CYP1B1) was recently shown to be a candidate tumor antigen broadly expressed in solid and hematologic malignancies. Nevertheless, use of such self‐antigens as targets for immune intervention can be limited because of loss of high‐avidity T cells during negative sele

Identification of HLA-A*0201-restricted
Identification of HLA-A*0201-restricted CTL epitopes encoded by the tumor-specific MAGE-2 gene product
✍ Marjan J.W. Visseren; Sjoerd H. van der Burg; Ellen I.H. van der Voort; Remco M. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 105 KB 👁 1 views

## MAGE -2 is expressed in many tumors, including melanoma, laryngeal tumors, lung tumors and sarcomas, but not in healthy tissue, with the exception of testis. Thus, MAGE-2derived peptides that bind to HLA class I molecules and elicit cytotoxic T lymphocyte (CTL) responses could be of significant

Identification of HLA-A3-restricted CD8+
Identification of HLA-A3-restricted CD8+ T cell epitopes derived from mammaglobin-A, a tumor-associated antigen of human breast cancer
✍ Andrés Jaramillo; Kanchana Majumder; Partha P. Manna; Timothy P. Fleming; Gerard 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 134 KB

## Abstract Mammaglobin‐A is highly overexpressed in breast cancer cell lines and primary breast tumors. This pattern of expression is restricted to mammary epithelium and metastatic breast tumors. Thus, mammaglobin‐A‐specific T cell immune responses may provide an important approach for the design