Isolation of E. coli promoters from the
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West, Robert W. ;McConnell, David ;Rodriguez, Raymond L.
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Article
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1980
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Springer
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English
β 957 KB
Promotor sequences recognized by Escherichia coli RNA polymerase have been isolated from bacteriophage T7 DNA using the plasmid pBRH4. T7 DNA was digested with the restriction endonuclease Hae III, Alu I, and Eco RI\* and the products of these digestions were ligated into the EcoRI site of pBRH4. Cl