MATERIALS AND METHODS ## Carboxy-p-amino[14C]benzoic acid was obtained from ICN and from Research Products International. Thionyl chloride was obtained from Matheson, Coleman and Bell, adenosine was obtained from Sigma, and hexamethylphosphoramide was obtained from Aldrich. Thin-layer chromatogr
โฆ LIBER โฆ
Identification of a lysine residue at a nucleotide binding site in the firefly luciferase with p-fluorosulfonyl[14C]benzoyl-5'-adenosine
โ Scribed by Lee, Yonih; Esch, Fred S.; DeLuca, Marlene A.
- Book ID
- 126017799
- Publisher
- American Chemical Society
- Year
- 1981
- Tongue
- English
- Weight
- 539 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0006-2960
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A simple three-step procedure is described for the purification of a labeled peptide from a tryptic digest of the P-subunit of the F,-ATPase after the enzyme had been inactivated with p-fluorosulfonyl-[14C]benzoyl-5'-adenosine. The procedure involves: (1) anion-exchange chromatography of a tryptic