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Identification of a 195 Kda protein in the striated rootlet: Its expression in ciliated and ciliogenic cells

✍ Scribed by Hagiwara, Haruo ;Aoki, Takeo ;Ohwada, Nobuo ;Fujimoto, Toyoshi

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 956 KB
Volume: 45
Category: Article
ISSN: 0886-1544
DOI: 10.1002/(sici)1097-0169(200003)45:3<200::aid-cm3>3.0.co;2-4

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✦ Synopsis

Little is known about the molecular composition of the ciliary rootlet. We raised monoclonal antibodies to a crude preparation of striated rootlets isolated from the human oviduct, and obtained a clone (R4109) that specifically labeled the ciliary rootlets. Rootlets associated with the solitary cilium in secretory cells and fibroblasts were also labeled. R4109 identified a 195-kDa protein by immunoblotting. Ciliogenic cells in the oviduct epithelium of young mice were labeled in the globular and/or granular pattern by R4109 by immunofluorescence microscopy. Immunoelectron microscopy showed that they corresponded to fibrogranular complex and dense granule, respectively. The result demonstrated that the 195-kDa protein is a component common to the striated rootlet and dense granule, and thus suggested that dense granules are involved in the rootlet formation. Cell

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