Identification and quantitation of 1,2-epoxybutene-3 as the primary metabolite of 1,3-butadiene

✍ Scribed by E. Malvoisin; G. Lhoest; F. Poncelet; M. Roberfroid; M. Mercier

• Book ID: 104144112
• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 450 KB
• Volume: 178
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)92500-7

✦ Synopsis

1,3-Butadiene was incubated in the presence of rat liver microsomes supplemented with an NADPH-generatin, y = s stem. One of the major metabolites of butadiene was found to be 1,2_epoxybutene-3, which was analysed by electron&pture gas-liquid chromatography after its derivatization with pentafluorophenylhydrazine. The effects of variation of several incubation parameters and of different pretreatments of the animals on its formation kinetics were evaluated. lNTRODUCIlON Butadiene is widely used as a polymer component in the manufacture of synthetic rubber and copolymeric plastics such as ABS (acjlonitrile-butadiene-styrene)'.

A previous report from our laboratory' has demonstrated that butadiene and 1,2_epoxybutene-3