Identification and quantification of glycoproteins by affinity electrophoresis

✍ Scribed by T.C. Bøg-Hansen; O.J. Bjerrum; C.-H. Brogren

• Book ID: 102984043
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 625 KB
• Volume: 81
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90600-5

✦ Synopsis

Affinity electrophoresis of lectin-binding glycoproteins in a gel containing lectin can be used for quantitation in analogy with crossed or rocket immunoelectrophoresis (electroimmunoassay). A correlation is found between the amount of glycoprotein applied and the rocket height. When glycoproteins are extensively denatured by heat, acid, or detergent, the rocket height is a measure of the amount of glycoprotein irrespective of the degree of denaturation.